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What defines biotic factors?

  1. Non-living things

  2. Living things

  3. Chemical substances

  4. Atmospheric conditions

The correct answer is: Living things

Biotic factors are defined as living things within an ecosystem, including plants, animals, fungi, bacteria, and other microorganisms. This is why option B is correct. Option A is incorrect because it refers to non-living things, which are known as abiotic factors. While abiotic factors do play a role in an ecosystem, they do not define biotic factors. Option C is also incorrect because chemical substances, such as water and oxygen, can be considered abiotic factors. Option D is incorrect because atmospheric conditions, while they may affect living things, are not considered living things themselves.